› 6mqw

X-ray diffraction
2.09Å resolution

Crystal Structure of All-trans Retinal-Bound R111K:Y134F:T54V:R132Q:P39Y:R59Y:L121E Human Cellular Retinoic Acid Binding Protein II Irradiated with 400 nm laser (30 seconds) and subsequently dark adapted (10 minutes) at 2.1 Angstrom Resolution

Released:
DOI: 10.2210/pdb6mqw/pdb
PDB entry 6mqw coloured by chain, front view.
PDB entry 6mqw coloured by chain, side view.
PDB entry 6mqw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mimicking Microbial Rhodopsin Isomerization in a Single Crystal.
Ghanbarpour A, Nairat M, Nosrati M, Santos EM, Vasileiou C, Dantus M, Borhan B, Geiger JH
J Am Chem Soc 141 1735-1741 (2019)
PMID: 30580520

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-151430 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cellular retinoic acid-binding protein 2 Chain: A
Molecule details ›
Chain: A
Length: 137 amino acids
Theoretical weight: 15.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P29373 (Residues: 2-138; Coverage: 99%)
Gene name: CRABP2
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

1 bound ligand:
Ligand RET 1 x RET
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P3121
Unit cell:
a: 58.586Å b: 58.586Å c: 100.178Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.183 0.22
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Rhodopsins: An Excitingly Versatile Protein Species for Research, Development and Creative Engineering.
de Grip et al. (2022)
6 other articles

1 mention without citation

Mimicking Microbial Rhodopsin Isomerization in a Single Crystal.
Ghanbarpour et al. (2019)