› 6mr4

X-ray diffraction
2.71Å resolution

Crystal structure of the Sth1 bromodomain from S.cerevisiae

Released:
DOI: 10.2210/pdb6mr4/pdb
PDB entry 6mr4 coloured by chain, front view.
PDB entry 6mr4 coloured by chain, side view.
PDB entry 6mr4 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Substrate Affinity and Specificity of the ScSth1p Bromodomain Are Fine-Tuned for Versatile Histone Recognition.
Blus BJ, Hashimoto H, Seo HS, Krolak A, Debler EW
Structure 27 1460-1468.e3 (2019)
PMID: 31327661

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-152385 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nuclear protein STH1/NPS1 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 112 amino acids
Theoretical weight: 13.35 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P32597 (Residues: 1250-1359; Coverage: 8%)
Gene names: NPS1, STH1, YIL126W
Sequence domains: Bromodomain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2
Unit cell:
a: 141.753Å b: 74.619Å c: 72.541Å
α: 90° β: 92.88° γ: 90°
R-values:
R R work R free
0.239 0.238 0.27
Expression system: Escherichia coli

Quick links