› 6muj

X-ray diffraction
2.25Å resolution

Formylglycine generating enzyme bound to copper

Released:
DOI: 10.2210/pdb6muj/pdb
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PDB entry 6muj coloured by chain, front view.
PDB entry 6muj coloured by chain, side view.
PDB entry 6muj coloured by chain, top view.
Source organism: Streptomyces coelicolor A3(2)
Primary publication:
Formylglycine-generating enzyme binds substrate directly at a mononuclear Cu(I) center to initiate O2 activation.
Appel MJ, Meier KK, Lafrance-Vanasse J, Lim H, Tsai CL, Hedman B, Hodgson KO, Tainer JA, Solomon EI, Bertozzi CR
Proc Natl Acad Sci U S A 116 5370-5375 (2019)
PMID: 30824597

Function and Biology Details

Reaction catalysed: 
A [sulfatase]-L-cysteine + O(2) + 2 a thiol = a [sulfatase]-3-oxo-L-alanine + hydrogen sulfide + a disulfide + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190248 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formylglycine-generating enzyme Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 319 amino acids
Theoretical weight: 34.28 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9F3C7 (Residues: 2-314; Coverage: 100%)
Gene name: SCO7548
Sequence domains: Sulfatase-modifying factor enzyme 1
Structure domains: paralog of FGE (formylglycine-generating enzyme)

Ligands and Environments

7 bound ligands:
Ligand CA 5 x CA
Ligand CU 20 x CU
Ligand IMD 4 x IMD
Ligand GOL 4 x GOL
Ligand FMT 1 x FMT
Ligand GLY 1 x GLY
Ligand DTT 1 x DTT
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: P3121
Unit cell:
a: 140.008Å b: 140.008Å c: 217.508Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.202 0.246
Expression system: Escherichia coli

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Citations

5 review citations

Sulfation pathways from red to green.
Günal et al. (2019)
4 more

3 mentions without citation

Formylglycine-generating enzyme binds substrate directly at a mononuclear Cu(I) center to initiate O2 activation.
Appel et al. (2019)
2 more

PDB-REDO

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