› 6mw9

Electron Microscopy
7.3Å resolution

CryoEM structure of chimeric Eastern Equine Encephalitis Virus with Fab of EEEV-3 antibody

Released:
DOI: 10.2210/pdb6mw9/pdb
PDB entry 6mw9 coloured by chain, front view.
PDB entry 6mw9 coloured by chain, side view.
PDB entry 6mw9 coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM Structures of Eastern Equine Encephalitis Virus Reveal Mechanisms of Virus Disassembly and Antibody Neutralization.
Hasan SS, Sun C, Kim AS, Watanabe Y, Chen CL, Klose T, Buda G, Crispin M, Diamond MS, Klimstra WB, Rossmann MG
OpenAccess logo Cell Rep 25 3136-3147.e5 (2018)
PMID: 30540945
Related structures: EMD-9274

Function and Biology Details

Reaction catalysed: 
Autocatalytic release of the core protein from the N-terminus of the togavirus structural polyprotein by hydrolysis of a -Trp-|-Ser- bond.
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 960-mer (preferred)
PDBe Complex ID:
PDB-CPX-207871 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Spike glycoprotein E1 Chains: A, E, I, M
Molecule details ›
Chains: A, E, I, M
Length: 441 amino acids
Theoretical weight: 47.94 KDa
Source organism: Eastern equine encephalitis virus
Expression system: Mesocricetus auratus
UniProt:
  • Canonical: Q4QXJ7 (Residues: 802-1242; Coverage: 36%)
Sequence domains: Alphavirus E1 glycoprotein
Spike glycoprotein E2 Chains: B, F, J, N
Molecule details ›
Chains: B, F, J, N
Length: 420 amino acids
Theoretical weight: 47.05 KDa
Source organism: Eastern equine encephalitis virus
Expression system: Mesocricetus auratus
UniProt:
  • Canonical: Q4QXJ7 (Residues: 325-744; Coverage: 34%)
Sequence domains: Alphavirus E2 glycoprotein
EEEV-3 antibody heavy chain Chains: C, G, K, O
Molecule details ›
Chains: C, G, K, O
Length: 218 amino acids
Theoretical weight: 23.74 KDa
Source organism: Mus musculus
Ig-like domain-containing protein Chains: D, H, L, P
Molecule details ›
Chains: D, H, L, P
Length: 214 amino acids
Theoretical weight: 23.18 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: G0YP42 (Residues: 20-233; Coverage: 100%)
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 7.3Å
Relevant EMDB volumes: EMD-9274
Expression system: Mesocricetus auratus

Quick links

Citations

7 review citations

A molecular understanding of alphavirus entry.
Holmes et al. (2020)
6 more

1 mention without citation

Cryo-EM Structures of Eastern Equine Encephalitis Virus Reveal Mechanisms of Virus Disassembly and Antibody Neutralization.
Hasan et al. (2018)