› 6myk

X-ray diffraction
1.8Å resolution

Pleurotus ostreatus OstreolysinA mutant E69A with Bis-Tris

Released:
DOI: 10.2210/pdb6myk/pdb
PDB entry 6myk coloured by chain, front view.
PDB entry 6myk coloured by chain, side view.
PDB entry 6myk coloured by chain, top view.
Source organism: Pleurotus ostreatus
Primary publication:
Molecular Discrimination between Two Conformations of Sphingomyelin in Plasma Membranes.
Endapally S, Frias D, Grzemska M, Gay A, Tomchick DR, Radhakrishnan A
Cell 176 1040-1053.e17 (2019)
PMID: 30712872

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-551160 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ostreolysin A6 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 138 amino acids
Theoretical weight: 15.02 KDa
Source organism: Pleurotus ostreatus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P83467 (Residues: 1-138; Coverage: 100%)
Gene name: OlyA6
Sequence domains: Aegerolysin
Structure domains: Mutm (Fpg) Protein; Chain: A, domain 2

Ligands and Environments

4 bound ligands:
Ligand EDO 8 x EDO
Ligand SO4 4 x SO4
Ligand NA 4 x NA
Ligand BTB 1 x BTB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 69.857Å b: 86.684Å c: 92.878Å
α: 90° β: 99.13° γ: 90°
R-values:
R R work R free
0.195 0.194 0.219
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Membrane Lipid Composition: Effect on Membrane and Organelle Structure, Function and Compartmentalization and Therapeutic Avenues.
Casares et al. (2019)
12 more

3 mentions without citation

Molecular Discrimination between Two Conformations of Sphingomyelin in Plasma Membranes.
Endapally et al. (2019)
2 more