› 6mzy

Electron Microscopy
3.3Å resolution

Cryo-EM structure of the HO BMC shell: Icosahedral reconstruction of the compacted subpopulation

Released:
DOI: 10.2210/pdb6mzy/pdb
PDB entry 6mzy coloured by chain, front view.
PDB entry 6mzy coloured by chain, side view.
PDB entry 6mzy coloured by chain, top view.
Source organism: Haliangium ochraceum DSM 14365
Primary publication:
The Plasticity of Molecular Interactions Governs Bacterial Microcompartment Shell Assembly.
Greber BJ, Sutter M, Kerfeld CA
Structure 27 749-763.e4 (2019)
PMID: 30833088
Related structures: EMD-9310

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 540-mer (preferred)
PDBe Complex ID:
PDB-CPX-504734 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Bacterial microcompartment shell vertex protein Chain: A1
Molecule details ›
Chain: A1
Length: 96 amino acids
Theoretical weight: 9.87 KDa
Source organism: Haliangium ochraceum DSM 14365
Expression system: Escherichia coli
UniProt:
  • Canonical: D0LHE5 (Residues: 1-96; Coverage: 100%)
Gene name: Hoch_5814
Sequence domains: Ethanolamine utilisation protein EutN/carboxysome
Bacterial microcompartment protein homohexamer Chains: A2, A3, A4, A5, A6, A7
Molecule details ›
Chains: A2, A3, A4, A5, A6, A7
Length: 99 amino acids
Theoretical weight: 10.13 KDa
Source organism: Haliangium ochraceum DSM 14365
Expression system: Escherichia coli
UniProt:
  • Canonical: D0LID5 (Residues: 1-99; Coverage: 100%)
Gene name: Hoch_5815
Sequence domains: BMC domain
Bacterial microcompartment protein trimer-2 Chains: A8, A9
Molecule details ›
Chains: A8, A9
Length: 212 amino acids
Theoretical weight: 22.9 KDa
Source organism: Haliangium ochraceum DSM 14365
Expression system: Escherichia coli
UniProt:
  • Canonical: D0LID6 (Residues: 1-212; Coverage: 100%)
Gene name: Hoch_5816
Sequence domains: BMC domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-9310
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Evolutionary relationships among shell proteins of carboxysomes and metabolosomes.
Melnicki et al. (2021)
6 more

1 mention without citation

The Plasticity of Molecular Interactions Governs Bacterial Microcompartment Shell Assembly.
Greber et al. (2019)