› 6n1y

X-ray diffraction
2.15Å resolution

Structure of L509V CAO1 - growth condition 1

Released:
DOI: 10.2210/pdb6n1y/pdb
PDB entry 6n1y coloured by chain, front view.
PDB entry 6n1y coloured by chain, side view.
PDB entry 6n1y coloured by chain, top view.
Source organism: Neurospora crassa
Primary publication:
Evidence for distinct rate-limiting steps in the cleavage of alkenes by carotenoid cleavage dioxygenases.
Khadka N, Farquhar ER, Hill HE, Shi W, von Lintig J, Kiser PD
J Biol Chem 294 10596-10606 (2019)
PMID: 31138651

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-245308 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carotenoid oxygenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 526 amino acids
Theoretical weight: 59.48 KDa
Source organism: Neurospora crassa
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand FE2 4 x FE2
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P3221
Unit cell:
a: 101.21Å b: 101.21Å c: 450.18Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.198 0.218
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Molecular components affecting ocular carotenoid and retinoid homeostasis.
von Lintig et al. (2021)
1 more

1 mention without citation

Evidence for distinct rate-limiting steps in the cleavage of alkenes by carotenoid cleavage dioxygenases.
Khadka et al. (2019)