› 6n2x

X-ray diffraction
3Å resolution

Anti-HIV-1 Fab 2G12 + Man9 re-refinement

Released:
DOI: 10.2210/pdb6n2x/pdb
Model geometry
Fit model/data
PDB entry 6n2x coloured by chain, front view.
PDB entry 6n2x coloured by chain, side view.
PDB entry 6n2x coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Antibody domain exchange is an immunological solution to carbohydrate cluster recognition.
Calarese DA, Scanlan CN, Zwick MB, Deechongkit S, Mimura Y, Kunert R, Zhu P, Wormald MR, Stanfield RL, Roux KH, Kelly JW, Rudd PM, Dwek RA, Katinger H, Burton DR, Wilson IA
Science 300 2065-71 (2003)
PMID: 12829775

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143690 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Immunoglobulin kappa light chain Chains: K, L
Molecule details ›
Chains: K, L
Length: 213 amino acids
Theoretical weight: 23.25 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P0DOX7 (Residues: 1-213; Coverage: 100%)
Sequence domains:
Structure domains: Immunoglobulins
Immunoglobulin gamma-1 heavy chain Chains: H, M
Molecule details ›
Chains: H, M
Length: 224 amino acids
Theoretical weight: 23.85 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P0DOX5 (Residues: 1-98, 99-220; Coverage: 49%)
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: I222
Unit cell:
a: 135.822Å b: 145.712Å c: 148.554Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.243 0.283
Expression system: Cricetulus griseus

Quick links

Citations

131 review citations

The impact of glycosylation on the biological function and structure of human immunoglobulins.
Arnold et al. (2007)
130 more

5 mentions without citation

Exploitation of glycosylation in enveloped virus pathobiology.
Watanabe et al. (2019)
4 more

PDB-REDO

The sliders below show the change in model quality between original PDB entry and the PDB-REDO entry

Model Geometry
 
 
Data not available
DNA/RNA Geometry
 
 
Data not available
Fit model/data
 
 
Data not available
PDB-REDO