› 6n5k

X-ray diffraction
3.1Å resolution

Structure of Human pir-miRNA-449c Apical Loop and One-base-pair Fused to the YdaO Riboswitch Scaffold

Released:
DOI: 10.2210/pdb6n5k/pdb
PDB entry 6n5k coloured by chain, front view.
PDB entry 6n5k coloured by chain, side view.
PDB entry 6n5k coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Shoffner GM, Peng Z, Guo F

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-250456 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
RNA (125-MER) Chain: A
Molecule details ›
Chain: A
Length: 125 nucleotides
Theoretical weight: 40.56 KDa
Source organism: Homo sapiens
Expression system: in vitro transcription vector pT7-Fluc(deltai)
Sequence domains: c-di-AMP riboswitch aptamer (ydaO/yuaA leader)

Ligands and Environments

4 bound ligands:
Ligand 2BA 2 x 2BA
Ligand MG 2 x MG
Ligand K 1 x K
Ligand SO4 1 x SO4
1 modified residue:
Ligand GTP 1 x GTP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P3121
Unit cell:
a: 113.96Å b: 113.96Å c: 114.83Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.177 0.186
Expression system: in vitro transcription vector pT7-Fluc(deltai)

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