› 6n71

X-ray diffraction
2.45Å resolution

Crystal Structure of ATPase delta1-79 Spa47 R191E

Released:
DOI: 10.2210/pdb6n71/pdb
PDB entry 6n71 coloured by chain, front view.
PDB entry 6n71 coloured by chain, side view.
PDB entry 6n71 coloured by chain, top view.
Source organism: Shigella flexneri
Primary publication:
Interfacial amino acids support Spa47 oligomerization and shigella type three secretion system activation.
Demler HJ, Case HB, Morales Y, Bernard AR, Johnson SJ, Dickenson NE
Proteins 87 931-942 (2019)
PMID: 31162724

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + cellular protein(Side 1) = ADP + phosphate + cellular protein(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140979 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type 3 secretion system ATPase Chains: A, B
Molecule details ›
Chains: A, B
Length: 351 amino acids
Theoretical weight: 38.98 KDa
Source organism: Shigella flexneri
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A1C1 (Residues: 80-430; Coverage: 82%)
Gene names: CP0149, mxiB, sctN, spa47, spaL
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 44.02Å b: 152.743Å c: 54.467Å
α: 90° β: 109.92° γ: 90°
R-values:
R R work R free
0.208 0.205 0.247
Expression system: Escherichia coli

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Citations

1 review citation

The T3SS of Shigella: Expression, Structure, Function, and Role in Vacuole Escape.
Bajunaid et al. (2020)
5 other articles