› 6n7h

Electron Microscopy
3.6Å resolution

Cryo-EM structure of the 2:1 hPtch1-Shhp complex

Released:
DOI: 10.2210/pdb6n7h/pdb
PDB entry 6n7h coloured by chain, front view.
PDB entry 6n7h coloured by chain, side view.
PDB entry 6n7h coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Inhibition of tetrameric Patched1 by Sonic Hedgehog through an asymmetric paradigm.
Qian H, Cao P, Hu M, Gao S, Yan N, Gong X
OpenAccess logo Nat Commun 10 2320 (2019)
PMID: 31127104
Related structures: EMD-0356

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171800 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Protein patched homolog 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 1349 amino acids
Theoretical weight: 150.19 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q13635 (Residues: 1-1305; Coverage: 90%)
Gene names: PTCH, PTCH1
Sequence domains: Patched family
Sonic hedgehog protein N-product Chain: C
Molecule details ›
Chain: C
Length: 174 amino acids
Theoretical weight: 19.59 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q15465 (Residues: 24-197; Coverage: 40%)
Gene name: SHH
Sequence domains: Hedgehog amino-terminal signalling domain

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
5 bound ligands:
Ligand NAG 10 x NAG
Ligand CLR 5 x CLR
Ligand ZN 1 x ZN
Ligand CA 2 x CA
Ligand PLM 1 x PLM
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-0356
Expression system: Homo sapiens

Quick links

Citations

21 review citations

Biochemical mechanisms of vertebrate hedgehog signaling.
Kong et al. (2019)
20 more

4 mentions without citation

Modulation of Hedgehog Signaling for the Treatment of Basal Cell Carcinoma and the Development of Preclinical Models.
Dukes et al. (2022)
3 more