› 6n7v

Electron Microscopy
3.8Å resolution

Structure of bacteriophage T7 gp4 (helicase-primase, E343Q mutant) in complex with ssDNA, dTTP, AC dinucleotide, and CTP (from multiple lead complexes)

Released:
DOI: 10.2210/pdb6n7v/pdb
PDB entry 6n7v coloured by chain, front view.
PDB entry 6n7v coloured by chain, side view.
PDB entry 6n7v coloured by chain, top view.
Source organism: Escherichia phage T7
Primary publication:
Structures and operating principles of the replisome.
Gao Y, Cui Y, Fox T, Lin S, Wang H, de Val N, Zhou ZH, Yang W
Science 363 (2019)
PMID: 30679383
Related structures: EMD-0364

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120324 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA helicase/primase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 566 amino acids
Theoretical weight: 62.73 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli
UniProt:
  • Canonical: P03692 (Residues: 1-566; Coverage: 100%)
Gene name: 4
Sequence domains:
DNA (93-MER) Chain: T
Molecule details ›
Chain: T
Length: 76 nucleotides
Theoretical weight: 23.28 KDa
Source organism: Escherichia phage T7
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand TTP 5 x TTP
Ligand MG 5 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-0364
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

20 review citations

Shaping human telomeres: from shelterin and CST complexes to telomeric chromatin organization.
Lim et al. (2021)
19 more

2 mentions without citation

Structures and operating principles of the replisome.
Gao et al. (2019)
1 more