› 6nbh

Electron Microscopy
3.5Å resolution

Cryo-EM structure of parathyroid hormone receptor type 1 in complex with a long-acting parathyroid hormone analog and G protein

Released:
DOI: 10.2210/pdb6nbh/pdb
PDB entry 6nbh coloured by chain, front view.
PDB entry 6nbh coloured by chain, side view.
PDB entry 6nbh coloured by chain, top view.
Source organisms:
Primary publication:
Structure and dynamics of the active human parathyroid hormone receptor-1.
Zhao LH, Ma S, Sutkeviciute I, Shen DD, Zhou XE, de Waal PW, Li CY, Kang Y, Clark LJ, Jean-Alphonse FG, White AD, Yang D, Dai A, Cai X, Chen J, Li C, Jiang Y, Watanabe T, Gardella TJ, Melcher K, Wang MW, Vilardaga JP, Xu HE, Zhang Y
Science 364 148-153 (2019)
PMID: 30975883
Related structures: EMD-0411

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-209130 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Parathyroid hormone/parathyroid hormone-related peptide receptor Chain: R
Molecule details ›
Chain: R
Length: 478 amino acids
Theoretical weight: 54.76 KDa
Source organism: Homo sapiens
Expression system: Spodoptera
UniProt:
  • Canonical: Q03431 (Residues: 27-502; Coverage: 84%)
Gene names: PTH1R, PTHR, PTHR1
Sequence domains:
Long-acting parathyroid hormone analog Chain: P
Molecule details ›
Chain: P
Length: 36 amino acids
Theoretical weight: 4.27 KDa
Source organism: Homo sapiens
Expression system: synthetic construct
Guanine nucleotide-binding protein G(i) subunit alpha-1; Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 378 amino acids
Theoretical weight: 43.87 KDa
Source organism: Bos taurus
Expression system: Spodoptera
UniProt:
  • Canonical: P04896 (Residues: 1-67, 208-394; Coverage: 65%)
  • Canonical: P63097 (Residues: 61-184; Coverage: 35%)
Gene names: GNAI1, GNAS, GNAS1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 345 amino acids
Theoretical weight: 37.92 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera
UniProt:
  • Canonical: P54311 (Residues: 2-340; Coverage: 100%)
Gene name: Gnb1
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Bos taurus
Expression system: Spodoptera
UniProt:
  • Canonical: P63212 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Structure domains: Transducin (heterotrimeric G protein), gamma chain
Nanobody-35 Chain: N
Molecule details ›
Chain: N
Length: 126 amino acids
Theoretical weight: 13.71 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand CLR 7 x CLR
Ligand PLM 3 x PLM
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-0411
Expression systems:
  • Spodoptera
  • synthetic construct
  • Escherichia coli

Quick links

Citations

16 review citations

Structural basis of GPCR coupling to distinct signal transducers: implications for biased signaling.
Seyedabadi et al. (2022)
15 more

8 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
7 more