› 6ncs

X-ray diffraction
1.8Å resolution

Crystal structure of N-acetylneuraminic acid (Sialic acid) synthetase from Leptospira borgpetersenii serovar Hardjo-bovis in complex with citrate

Released:
DOI: 10.2210/pdb6ncs/pdb
PDB entry 6ncs coloured by chain, front view.
PDB entry 6ncs coloured by chain, side view.
PDB entry 6ncs coloured by chain, top view.
Source organism: Leptospira borgpetersenii serovar Hardjo-bovis str. JB197
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170116 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetylneuraminic acid synthase N-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 290 amino acids
Theoretical weight: 32.29 KDa
Source organism: Leptospira borgpetersenii serovar Hardjo-bovis str. JB197
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q04TM5 (Residues: 1-282; Coverage: 100%)
Gene name: LBJ_1133
Sequence domains: NeuB family

Ligands and Environments

6 bound ligands:
Ligand MN 2 x MN
Ligand CIT 2 x CIT
Ligand ACT 1 x ACT
Ligand FMT 1 x FMT
Ligand TAR 1 x TAR
Ligand IOD 20 x IOD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P21
Unit cell:
a: 69.88Å b: 49.12Å c: 88.85Å
α: 90° β: 112.41° γ: 90°
R-values:
R R work R free
0.157 0.156 0.198
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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