› 6nd4

Electron Microscopy
4.3Å resolution

Conformational switches control early maturation of the eukaryotic small ribosomal subunit

Released:
DOI: 10.2210/pdb6nd4/pdb
PDB entry 6nd4 coloured by chain, front view.
PDB entry 6nd4 coloured by chain, side view.
PDB entry 6nd4 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae BY4741
Primary publication:
Conformational switches control early maturation of the eukaryotic small ribosomal subunit.
Hunziker M, Barandun J, Buzovetsky O, Steckler C, Molina H, Klinge S
OpenAccess logo Elife 8 (2019)
PMID: 31206356
Related structures: EMD-0441

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 30-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-294011 (preferred)
Entry contents:
25 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (28 distinct):
NET1-associated nuclear protein 1 Chain: H
Molecule details ›
Chain: H
Length: 853 amino acids
Theoretical weight: 96.36 KDa
Source organism: Saccharomyces cerevisiae BY4741
UniProt:
  • Canonical: Q02931 (Residues: 1-896; Coverage: 95%)
Gene names: NAN1, UTP17, YPL126W
Sequence domains: WD domain, G-beta repeat
U3 small nucleolar RNA-associated protein 8 Chain: I
Molecule details ›
Chain: I
Length: 519 amino acids
Theoretical weight: 49.06 KDa
Source organism: Saccharomyces cerevisiae BY4741
UniProt:
  • Canonical: P53276 (Residues: 549-713; Coverage: 23%)
Gene names: UTP8, YGR128C
Sequence domains: Utp8, C-terminal
U3 small nucleolar RNA-associated protein 15 Chain: J
U3 small nucleolar RNA-associated protein 9 Chain: K
U3 small nucleolar RNA-associated protein 5 Chain: L
U3 small nucleolar RNA-associated protein 10 Chain: M
U3 small nucleolar RNA-associated protein 4 Chain: N
Periodic tryptophan protein 2 Chain: O
U3 small nucleolar RNA-associated protein 6 Chain: P
U3 small nucleolar RNA-associated protein 12 Chain: Q
U3 small nucleolar RNA-associated protein 13 Chain: R
U3 small nucleolar RNA-associated protein 18 Chain: S
U3 small nucleolar RNA-associated protein 21 Chain: T
Protein SOF1 Chain: U
U3 small nucleolar RNA-associated protein 7 Chain: W
U3 small nucleolar ribonucleoprotein protein IMP3 Chain: Z
U3 small nucleolar RNA-associated protein MPP10 Chain: A
U3 small nucleolar RNA-associated protein 16 Chain: D
Nucleolar protein 56 Chain: a
Nucleolar protein 58 Chain: b
rRNA 2'-O-methyltransferase fibrillarin Chains: c, d
13 kDa ribonucleoprotein-associated protein Chains: e, f
Ribosomal RNA-processing protein 9 Chain: g
rRNA-processing protein FCF1 Chain: l
Unidentified fragment Chain: x
5'ETS rRNA Chain: 0
18S rRNA 5' domain start Chain: 1
U3 snoRNA Chain: 2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 4.3Å
Relevant EMDB volumes: EMD-0441

Quick links

Citations

4 review citations

RNA folding and functions of RNA helicases in ribosome biogenesis.
Mitterer et al. (2022)
3 more

3 mentions without citation

Conformational switches control early maturation of the eukaryotic small ribosomal subunit.
Hunziker et al. (2019)
2 more