› 6nfy

X-ray diffraction
2.17Å resolution

Crystal structure of nonphosphorylated, HPK1 kinase domain in complex with sunitinib in the inactive state.

Released:
DOI: 10.2210/pdb6nfy/pdb
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PDB entry 6nfy coloured by chain, front view.
PDB entry 6nfy coloured by chain, side view.
PDB entry 6nfy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Multiple conformational states of the HPK1 kinase domain in complex with sunitinib reveal the structural changes accompanying HPK1 trans-regulation.
Johnson E, McTigue M, Gallego RA, Johnson TW, Timofeevski S, Maestre M, Fisher TS, Kania R, Sawasdikosol S, Burakoff S, Cronin CN
J Biol Chem 294 9029-9036 (2019)
PMID: 31018963

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187814 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitogen-activated protein kinase kinase kinase kinase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 300 amino acids
Theoretical weight: 33.4 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q92918 (Residues: 10-307; Coverage: 36%)
Gene names: HPK1, MAP4K1
Sequence domains: Protein kinase domain

Ligands and Environments

1 bound ligand:
Ligand B49 2 x B49
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 17-ID
Spacegroup: R32
Unit cell:
a: 165.908Å b: 165.908Å c: 163.583Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.192 0.219
Expression system: Spodoptera frugiperda

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Citations

1 review citation

A perspective on HPK1 as a novel immuno-oncology drug target.
Sawasdikosol et al. (2020)
7 other articles

3 mentions without citation

Multiple conformational states of the HPK1 kinase domain in complex with sunitinib reveal the structural changes accompanying HPK1 trans-regulation.
Johnson et al. (2019)
2 more

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