6nhv

Electron Microscopy
3.5Å resolution

Single particle reconstruction of DARPin and its bound GFP on a symmetric scaffold

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-167129 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
superfolder GFP Chain: A
Molecule details ›
Chain: A
Length: 234 amino acids
Theoretical weight: 26.62 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
5-carboxymethyl-2-hydroxymuconate isomerase Chains: N, O, X
Molecule details ›
Chains: N, O, X
Length: 131 amino acids
Theoretical weight: 14.35 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I2D8 (Residues: 1-123; Coverage: 100%)
Gene name: PA1966
Sequence domains: 5-carboxymethyl-2-hydroxymuconate isomerase
Structure domains: Macrophage Migration Inhibitory Factor
Subunit A-DARPin Chains: R, S, T
Molecule details ›
Chains: R, S, T
Length: 319 amino acids
Theoretical weight: 34.72 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-9374
Expression system: Escherichia coli