6njn

Electron Microscopy
6.5Å resolution

Architecture and subunit arrangement of native AMPA receptors

Released:
Source organisms:
Related structures: EMD-9389

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
PDBe Complex ID:
PDB-CPX-210752 (preferred)
Entry contents:
9 distinct polypeptide molecules
Macromolecules (11 distinct):
Glutamate receptor 1 Chain: A
Molecule details ›
Chain: A
Length: 907 amino acids
Theoretical weight: 101.52 KDa
Source organism: Rattus norvegicus
UniProt:
  • Canonical: P19490 (Residues: 1-907; Coverage: 100%)
Gene names: GluA1, Glur1, Gria1
Sequence domains:
Glutamate receptor 2 Chains: B, D
Molecule details ›
Chains: B, D
Length: 883 amino acids
Theoretical weight: 98.78 KDa
Source organism: Rattus norvegicus
UniProt:
  • Canonical: P19491 (Residues: 1-883; Coverage: 100%)
Gene names: GluA2, Glur2, Gria2
Sequence domains:
Glutamate receptor 3 Chain: C
Molecule details ›
Chain: C
Length: 888 amino acids
Theoretical weight: 100.56 KDa
Source organism: Rattus norvegicus
UniProt:
  • Canonical: P19492 (Residues: 1-888; Coverage: 100%)
  • Best match: P19492-2 (Residues: 1-888)
Gene names: GluA3, Glur3, Gria3
Sequence domains:
A'-C' auxiliary proteins Chains: E, G
Molecule details ›
Chains: E, G
Length: 153 amino acids
Theoretical weight: 13.04 KDa
Source organism: Rattus norvegicus
Voltage-dependent calcium channel gamma-2 subunit Chains: F, H
Molecule details ›
Chains: F, H
Length: 323 amino acids
Theoretical weight: 35.94 KDa
Source organism: Rattus norvegicus
UniProt:
  • Canonical: Q71RJ2 (Residues: 1-323; Coverage: 100%)
Gene names: Cacng2, Stg
Sequence domains: PMP-22/EMP/MP20/Claudin family
11B8 scFv Chain: I
Molecule details ›
Chain: I
Length: 257 amino acids
Theoretical weight: 27.51 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
15F1 Fab light chain Chains: J, N
Molecule details ›
Chains: J, N
Length: 225 amino acids
Theoretical weight: 25.11 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
15F1 Fab heavy chain Chains: K, O
Molecule details ›
Chains: K, O
Length: 262 amino acids
Theoretical weight: 27.98 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
5B2 Fab Chains: L, M
Molecule details ›
Chains: L, M
Length: 224 amino acids
Theoretical weight: 19.08 KDa
Source organism: Rattus norvegicus

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer : NEW Components: NAG
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.5Å
Relevant EMDB volumes: EMD-9389
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda