› 6nl6

X-ray diffraction
1.4Å resolution

Crystal structure of mutant B1 immunoglobulin-binding domain of Streptococcal Protein G (T16F, T18A, V21E, T25L, K28Y, V29I, K31R, Q32H, Y33L, N35K, D36H, N37Q)

Released:
DOI: 10.2210/pdb6nl6/pdb
PDB entry 6nl6 coloured by chain, front view.
PDB entry 6nl6 coloured by chain, side view.
PDB entry 6nl6 coloured by chain, top view.
Source organism: Streptococcus
Primary publication:
Design of High-Affinity Metal-Controlled Protein Dimers.
Maniaci B, Lipper CH, Anipindi DL, Erlandsen H, Cole JL, Stec B, Huxford T, Love JJ
Biochemistry 58 2199-2207 (2019)
PMID: 30938154

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin G-binding protein G Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 56 amino acids
Theoretical weight: 6.35 KDa
Source organism: Streptococcus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19909 (Residues: 303-357; Coverage: 10%)
Gene name: spg
Sequence domains: B domain
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

2 bound ligands:
Ligand ZN 6 x ZN
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P32
Unit cell:
a: 48.292Å b: 48.292Å c: 83.341Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.149 0.145 0.214
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Biofabrication of functional protein nanoparticles through simple His-tag engineering.
López-Laguna et al. (2021)