› 6nmt

X-ray diffraction
1.83Å resolution

Non-Blocking Fab 3 anti-SIRP-alpha antibody in complex with SIRP-alpha Variant 1

Released:
DOI: 10.2210/pdb6nmt/pdb
Model geometry
Fit model/data
PDB entry 6nmt coloured by chain, front view.
PDB entry 6nmt coloured by chain, side view.
PDB entry 6nmt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Discovery of high affinity, pan-allelic, and pan-mammalian reactive antibodies against the myeloid checkpoint receptor SIRPα.
Sim J, Sockolosky JT, Sangalang E, Izquierdo S, Pedersen D, Harriman W, Wibowo AS, Carter J, Madan A, Doyle L, Harrabi O, Kauder SE, Chen A, Kuo TC, Wan H, Pons J
OpenAccess logo MAbs 11 1036-1052
PMID: 31257988

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211517 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab 3 anti-SIRP-alpha antibody Variable Light Chain Chain: A
Molecule details ›
Chain: A
Length: 209 amino acids
Theoretical weight: 22.42 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Fab 3 anti-SIRP-alpha antibody Variable Heavy Chain Chain: B
Molecule details ›
Chain: B
Length: 228 amino acids
Theoretical weight: 23.88 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Tyrosine-protein phosphatase non-receptor type substrate 1 Chain: C

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: I422
Unit cell:
a: 126.37Å b: 126.37Å c: 229.67Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.178 0.204
Expression system: Homo sapiens

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Citations

14 review citations

The CD47-SIRPα Immune Checkpoint.
Logtenberg et al. (2020)
13 more

2 mentions without citation

Discovery of high affinity, pan-allelic, and pan-mammalian reactive antibodies against the myeloid checkpoint receptor SIRPα.
Sim et al. (2019)
1 more

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