› 6npz

X-ray diffraction
2.12Å resolution

Crystal structure of Akt1 (aa 123-480) kinase with a bisubstrate

Released:
DOI: 10.2210/pdb6npz/pdb
PDB entry 6npz coloured by chain, front view.
PDB entry 6npz coloured by chain, side view.
PDB entry 6npz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Akt Kinase Activation Mechanisms Revealed Using Protein Semisynthesis.
Chu N, Salguero AL, Liu AZ, Chen Z, Dempsey DR, Ficarro SB, Alexander WM, Marto JA, Li Y, Amzel LM, Gabelli SB, Cole PA
Cell 174 897-907.e14 (2018)
PMID: 30078705

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-542519 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RAC-alpha serine/threonine-protein kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 358 amino acids
Theoretical weight: 41.39 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P31749 (Residues: 123-480; Coverage: 75%)
Gene names: AKT1, PKB, RAC
Sequence domains:
Structure domains:
bisubstrate Chains: F, G
Molecule details ›
Chains: F, G
Length: 10 amino acids
Theoretical weight: 1.69 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda

Ligands and Environments

4 bound ligands:
Ligand MN 4 x MN
Ligand PGE 2 x PGE
Ligand GOL 1 x GOL
Ligand VO4 1 x VO4
2 modified residues:
Ligand TPO 2 x TPO
Ligand SEP 4 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-1
Spacegroup: P21
Unit cell:
a: 86.321Å b: 56.088Å c: 92.019Å
α: 90° β: 104.56° γ: 90°
R-values:
R R work R free
0.188 0.185 0.241
Expression system: Spodoptera frugiperda

Quick links

Citations

17 review citations

Regulation of mTORC2 Signaling.
Fu et al. (2020)
16 more

17 mentions without citation

Computer-Aided Identification of Kinase-Targeted Small Molecules for Cancer: A Review on AKT Protein.
Primavera et al. (2023)
16 more