6nqd

Electron Microscopy
3.9Å resolution

Cryo-EM structure of T/F100 SOSIP.664 HIV-1 Env trimer in complex with 8ANC195 Fab

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-278948 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (9 distinct):
Envelope glycoprotein gp160 Chains: A, E, I
Molecule details ›
Chains: A, E, I
Length: 486 amino acids
Theoretical weight: 54.74 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: A0A140EMT3 (Residues: 29-508; Coverage: 56%)
Gene name: env
Sequence domains: Envelope glycoprotein GP120
Transmembrane protein gp41 Chains: B, F, J
Molecule details ›
Chains: B, F, J
Length: 184 amino acids
Theoretical weight: 20.33 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: A0A140EMT3 (Residues: 513-665; Coverage: 18%)
Gene name: env
Sequence domains: Retroviral envelope protein
Ig-like domain-containing protein Chains: C, G, K
Molecule details ›
Chains: C, G, K
Length: 244 amino acids
Theoretical weight: 26.15 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: S6B2A6 (Residues: 20-45, 46-91, 92-121, 122-124, 125-251; Coverage: 82%)
Sequence domains:
Structure domains: Immunoglobulins
Immunoglobulin kappa light chain Chains: D, H, L
Molecule details ›
Chains: D, H, L
Length: 215 amino acids
Theoretical weight: 23.46 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P0DOX7 (Residues: 1-30, 31-214; Coverage: 100%)
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-0485
Expression system: Homo sapiens