› 6nqt

X-ray diffraction
3.05Å resolution

GalNac-T2 soaked with UDP-sugar

Released:
DOI: 10.2210/pdb6nqt/pdb
Model geometry
Fit model/data
PDB entry 6nqt coloured by chain, front view.
PDB entry 6nqt coloured by chain, side view.
PDB entry 6nqt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Bump-and-Hole Engineering Identifies Specific Substrates of Glycosyltransferases in Living Cells.
Schumann B, Malaker SA, Wisnovsky SP, Debets MF, Agbay AJ, Fernandez D, Wagner LJS, Lin L, Li Z, Choi J, Fox DM, Peh J, Gray MA, Pedram K, Kohler JJ, Mrksich M, Bertozzi CR
OpenAccess logo Mol Cell 78 824-834.e15 (2020)
PMID: 32325029

Function and Biology Details

Reaction catalysed: 
UDP-N-acetyl-alpha-D-galactosamine + [protein]-L-serine = UDP + [protein]-3-O-(N-acetyl-alpha-D-galactosaminyl)-L-serine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171230 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polypeptide N-acetylgalactosaminyltransferase 2 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 571 amino acids
Theoretical weight: 64.74 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q10471 (Residues: 1-571; Coverage: 100%)
Gene name: GALNT2
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand MN 6 x MN
Ligand LR7 6 x LR7
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 116.575Å b: 120.126Å c: 247.389Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.231 0.286
Expression system: Homo sapiens

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Citations

14 review citations

A Pragmatic Guide to Enrichment Strategies for Mass Spectrometry-Based Glycoproteomics.
Riley et al. (2021)
13 more

3 mentions without citation

Generating orthogonal glycosyltransferase and nucleotide sugar pairs as next-generation glycobiology tools.
Cioce et al. (2021)
2 more

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