› 6nsk

Electron Microscopy
2.7Å resolution

CryoEM structure of Helicobacter pylori urea channel in open state.

Released:
DOI: 10.2210/pdb6nsk/pdb
PDB entry 6nsk coloured by chain, front view.
PDB entry 6nsk coloured by chain, side view.
PDB entry 6nsk coloured by chain, top view.
Source organism: Helicobacter pylori J99
Primary publication:
pH-dependent gating mechanism of the Helicobacter pylori urea channel revealed by cryo-EM.
Cui Y, Zhou K, Strugatsky D, Wen Y, Sachs G, Zhou ZH, Munson K
OpenAccess logo Sci Adv 5 eaav8423 (2019)
PMID: 30906870
Related structures: EMD-0499

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157573 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acid-activated urea channel Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 201 amino acids
Theoretical weight: 22.53 KDa
Source organism: Helicobacter pylori J99
Expression system: Escherichia coli
UniProt:
  • Canonical: P56874 (Residues: 1-61, 62-195; Coverage: 100%)
Gene names: jhp_0066, ureI
Sequence domains: AmiS/UreI family transporter
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat

Ligands and Environments

1 bound ligand:
Ligand XP4 18 x XP4
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.7Å
Relevant EMDB volumes: EMD-0499
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Cryo-electron microscopy analysis of small membrane proteins.
Nygaard et al. (2020)
5 more