› 6nt4

Electron Microscopy
3.5Å resolution

Cryo-EM structure of a human-cockroach hybrid Nav channel bound to alpha-scorpion toxin AaH2.

Released:
DOI: 10.2210/pdb6nt4/pdb
PDB entry 6nt4 coloured by chain, front view.
PDB entry 6nt4 coloured by chain, side view.
PDB entry 6nt4 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of α-scorpion toxin action on Nav channels.
Clairfeuille T, Cloake A, Infield DT, Llongueras JP, Arthur CP, Li ZR, Jian Y, Martin-Eauclaire MF, Bougis PE, Ciferri C, Ahern CA, Bosmans F, Hackos DH, Rohou A, Payandeh J
Science 363 (2019)
PMID: 30733386
Related structures: EMD-0501

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111948 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Sodium channel protein PaFPC1; Sodium channel protein type 9 subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 1559 amino acids
Theoretical weight: 178.76 KDa
Source organisms: Expression system: Homo sapiens
UniProt:
  • Canonical: D0E0C2 (Residues: 1-269, 289-1155, 1287-1505; Coverage: 87%)
  • Canonical: Q15858 (Residues: 257-275, 1501-1631; Coverage: 8%)
Gene names: NENA, SCN9A
Sequence domains:
Alpha-mammal toxin AaH2 Chains: B, C
Molecule details ›
Chains: B, C
Length: 65 amino acids
Theoretical weight: 7.26 KDa
Source organism: Androctonus australis
UniProt:
  • Canonical: P01484 (Residues: 20-83; Coverage: 97%)
Sequence domains: Scorpion toxin-like domain
Structure domains: Knottin, scorpion toxin-like

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
5 bound ligands:
Ligand NAG 5 x NAG
Ligand 76F 5 x 76F
Ligand AJP 1 x AJP
Ligand LHG 1 x LHG
Ligand Y01 1 x Y01
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-0501
Expression system: Homo sapiens

Quick links

Citations

11 review citations

Epilepsy-Related Voltage-Gated Sodium Channelopathies: A Review.
Menezes et al. (2020)
10 more

8 mentions without citation

Structures Illuminate Cardiac Ion Channel Functions in Health and in Long QT Syndrome.
Brewer et al. (2020)
7 more