› 6nvw

X-ray diffraction
2.2Å resolution

Crystal structure of penicillin G acylase from Bacillus megaterium

Released:
DOI: 10.2210/pdb6nvw/pdb
PDB entry 6nvw coloured by chain, front view.
PDB entry 6nvw coloured by chain, side view.
PDB entry 6nvw coloured by chain, top view.
Source organism: Priestia megaterium
Primary publication:
Crystal structures and protein engineering of three different penicillin G acylases from Gram-positive bacteria with different thermostability.
Mayer J, Pippel J, Günther G, Müller C, Lauermann A, Knuuti T, Blankenfeldt W, Jahn D, Biedendieck R
Appl Microbiol Biotechnol 103 7537-7552 (2019)
PMID: 31227867

Function and Biology Details

Reaction catalysed: 
Penicillin + H(2)O = a carboxylate + 6-aminopenicillanate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-562961 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Penicillin G acylase subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 210 amino acids
Theoretical weight: 24.45 KDa
Source organism: Priestia megaterium
Expression system: Priestia megaterium
UniProt:
  • Canonical: Q60136 (Residues: 25-234; Coverage: 27%)
Gene names: pac, pga
Penicillin G acylase subunit beta Chain: B
Molecule details ›
Chain: B
Length: 537 amino acids
Theoretical weight: 61.48 KDa
Source organism: Priestia megaterium
Expression system: Priestia megaterium
UniProt:
  • Canonical: Q60136 (Residues: 266-802; Coverage: 69%)
Gene names: pac, pga
Sequence domains: Penicillin amidase

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 58.238Å b: 77.961Å c: 84.086Å
α: 90° β: 101.67° γ: 90°
R-values:
R R work R free
0.218 0.215 0.275
Expression system: Priestia megaterium

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Citations

1 review citation

The "beauty in the beast"-the multiple uses of Priestia megaterium in biotechnology.
Biedendieck et al. (2021)
2 other articles