› 6nwg

X-ray diffraction
1.94Å resolution

Crystal structure of Mycobacterium tuberculosis dethiobiotin synthetase in complex with fragment analogue 3a

Released:
DOI: 10.2210/pdb6nwg/pdb
PDB entry 6nwg coloured by chain, front view.
PDB entry 6nwg coloured by chain, side view.
PDB entry 6nwg coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Entry authors: Thompson AP, Polyak SW, Wegener KL, Bruning JB

Function and Biology Details

Reaction catalysed: 
ATP + 7,8-diaminononanoate + CO(2) = ADP + phosphate + dethiobiotin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162006 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dethiobiotin synthetase BioD Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 233 amino acids
Theoretical weight: 23.29 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WPQ5 (Residues: 2-226; Coverage: 100%)
Gene names: MTCY336.33c, Rv1570, bioD
Sequence domains: AAA domain

Ligands and Environments

1 bound ligand:
Ligand L6J 3 x L6J
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 56.322Å b: 106.879Å c: 152.761Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.243
Expression system: Escherichia coli BL21(DE3)

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