› 6nwl

X-ray diffraction
1.6Å resolution

Structure of the Ancestral Glucocorticoid Receptor 2 ligand binding domain in complex with hydrocortisone and PGC1a coregulator fragment

Released:
DOI: 10.2210/pdb6nwl/pdb
PDB entry 6nwl coloured by chain, front view.
PDB entry 6nwl coloured by chain, side view.
PDB entry 6nwl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
First High-Resolution Crystal Structures of the Glucocorticoid Receptor Ligand-Binding Domain-Peroxisome Proliferator-Activated γ Coactivator 1-α Complex with Endogenous and Synthetic Glucocorticoids.
Liu X, Wang Y, Ortlund EA
Mol Pharmacol 96 408-417 (2019)
PMID: 31391291

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167162 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
glucocorticoid receptor Chain: A
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 28.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Peroxisome proliferator-activated receptor gamma coactivator 1-alpha Chain: B
Molecule details ›
Chain: B
Length: 12 amino acids
Theoretical weight: 1.27 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

5 bound ligands:
Ligand HCY 1 x HCY
Ligand GOL 3 x GOL
Ligand TLA 1 x TLA
Ligand EPE 1 x EPE
Ligand SCN 2 x SCN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2221
Unit cell:
a: 71.756Å b: 96.435Å c: 108.195Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.179 0.193
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Structural insights into glucocorticoid receptor function.
Frank et al. (2021)
2 more

2 mentions without citation

First High-Resolution Crystal Structures of the Glucocorticoid Receptor Ligand-Binding Domain-Peroxisome Proliferator-Activated γ Coactivator 1-α Complex with Endogenous and Synthetic Glucocorticoids.
Liu et al. (2019)
1 more