› 6o0q

X-ray diffraction
1.8Å resolution

Crystal structure of the TIR domain from human SARM1 in complex with ribose

Released:
DOI: 10.2210/pdb6o0q/pdb
PDB entry 6o0q coloured by chain, front view.
PDB entry 6o0q coloured by chain, side view.
PDB entry 6o0q coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
NAD+ cleavage activity by animal and plant TIR domains in cell death pathways.
Horsefield S, Burdett H, Zhang X, Manik MK, Shi Y, Chen J, Qi T, Gilley J, Lai JS, Rank MX, Casey LW, Gu W, Ericsson DJ, Foley G, Hughes RO, Bosanac T, von Itzstein M, Rathjen JP, Nanson JD, Boden M, Dry IB, Williams SJ, Staskawicz BJ, Coleman MP, Ve T, Dodds PN, Kobe B
Science 365 793-799 (2019)
PMID: 31439792

Function and Biology Details

Reaction catalysed: 
(1a) NAD(+) = cyclic ADP-ribose + nicotinamide
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-564528 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD(+) hydrolase SARM1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 144 amino acids
Theoretical weight: 16.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6SZW1 (Residues: 560-700; Coverage: 20%)
Gene names: KIAA0524, SAMD2, SARM, SARM1
Sequence domains: TIR domain

Ligands and Environments

2 bound ligands:
Ligand BDR 2 x BDR
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Unit cell:
a: 32.986Å b: 86.019Å c: 116.684Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.21
Expression system: Escherichia coli

Quick links

Citations

49 review citations

Programmed axon degeneration: from mouse to mechanism to medicine.
Coleman et al. (2020)
48 more

11 mentions without citation

Emergence of SARM1 as a Potential Therapeutic Target for Wallerian-type Diseases.
Loring et al. (2020)
10 more