› 6o2v

X-ray diffraction
1.58Å resolution

Crystal structure of the SARAF luminal domain Cys-lock mutant monomer

Released:
DOI: 10.2210/pdb6o2v/pdb
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PDB entry 6o2v coloured by chain, front view.
PDB entry 6o2v coloured by chain, side view.
PDB entry 6o2v coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
SARAF Luminal Domain Structure Reveals a Novel Domain-Swapped β-Sandwich Fold Important for SOCE Modulation.
Kimberlin CR, Meshcheriakova A, Palty R, Raveh A, Karbat I, Reuveny E, Minor DL
J Mol Biol 431 2869-2883 (2019)
PMID: 31082439

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188388 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Store-operated calcium entry-associated regulatory factor Chains: A, B
Molecule details ›
Chains: A, B
Length: 135 amino acids
Theoretical weight: 15.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96BY9 (Residues: 30-164; Coverage: 43%)
Gene names: HSPC035, NPD003, PSEC0019, SARAF, TMEM66, UNQ1967/PRO4499, XTP3
Sequence domains: SOCE-associated regulatory factor of calcium homoeostasis

Ligands and Environments

2 bound ligands:
Ligand FMT 1 x FMT
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 59.689Å b: 60.953Å c: 63.441Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.165 0.203
Expression system: Escherichia coli

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Citations

5 review citations

Structural Insights into the Mechanisms and Pharmacology of K2P Potassium Channels.
Natale et al. (2021)
4 more

2 mentions without citation

Exploring protein functions from structural flexibility using CABS-flex modeling.
Nithin et al. (2024)
1 more

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