› 6o5m

X-ray diffraction
2.3Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-236990 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tubulin alpha-1B chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 450 amino acids
Theoretical weight: 50.04 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: Q2XVP4 (Residues: 1-450; Coverage: 100%)
Gene name: TUBA1B
Sequence domains:
Tubulin beta chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 445 amino acids
Theoretical weight: 50 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P02554 (Residues: 1-445; Coverage: 100%)
Sequence domains:
Stathmin-4 Chain: E
Tubulin Tyrosine Ligase Chain: F

Ligands and Environments

8 bound ligands:
Ligand GTP 3 x GTP
Ligand CA 2 x CA
Ligand MG 4 x MG
Ligand GOL 2 x GOL
Ligand GDP 1 x GDP
Ligand MES 2 x MES
Ligand G8K 2 x G8K
Ligand ACP 1 x ACP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 104.966Å b: 157.721Å c: 181.615Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.223
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Colchicine-Binding Site Inhibitors from Chemistry to Clinic: A Review.
McLoughlin et al. (2020)
5 more

1 mention without citation

Molecular interactions at the colchicine binding site in tubulin: An X-ray crystallography perspective.
Wang et al. (2022)

PDB-REDO

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