› 6o86

X-ray diffraction
1.8Å resolution

Crystal Structure of SeMet UDP-dependent glucosyltransferases (UGT) from Stevia rebaudiana in complex with UDP

Released:
DOI: 10.2210/pdb6o86/pdb
PDB entry 6o86 coloured by chain, front view.
PDB entry 6o86 coloured by chain, side view.
PDB entry 6o86 coloured by chain, top view.
Source organism: Stevia rebaudiana
Primary publication:
Molecular basis for branched steviol glucoside biosynthesis.
Lee SG, Salomon E, Yu O, Jez JM
Proc Natl Acad Sci U S A 116 13131-13136 (2019)
PMID: 31182573

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-564625 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-glycosyltransferase 76G1 Chain: A
Molecule details ›
Chain: A
Length: 458 amino acids
Theoretical weight: 52.09 KDa
Source organism: Stevia rebaudiana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6VAB4 (Residues: 1-458; Coverage: 100%)
Gene name: UGT76G1
Sequence domains: UDP-glucoronosyl and UDP-glucosyl transferase

Ligands and Environments

1 bound ligand:
Ligand UDP 1 x UDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3121
Unit cell:
a: 97.982Å b: 97.982Å c: 90.962Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.174 0.173 0.196
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Structure-function and engineering of plant UDP-glycosyltransferase.
Wang et al. (2023)
8 other articles

3 mentions without citation

Molecular basis for branched steviol glucoside biosynthesis.
Lee et al. (2019)
2 more