› 6oa6

X-ray diffraction
1.37Å resolution

CRYSTAL STRUCTURE OF THE ACTIN-BINDING DOMAIN OF HUMAN ALPHA-ACTININ-4

Released:
DOI: 10.2210/pdb6oa6/pdb
PDB entry 6oa6 coloured by chain, front view.
PDB entry 6oa6 coloured by chain, side view.
PDB entry 6oa6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Phosphorylation of ACTN4 Leads to Podocyte Vulnerability and Proteinuric Glomerulosclerosis.
Feng D, Kumar M, Muntel J, Gurley SB, Birrane G, Stillman IE, Ding L, Wang M, Ahmed S, Schlondorff J, Alper SL, Ferrante T, Marquez SL, Ng CF, Novak R, Ingber DE, Steen H, Pollak MR
J Am Soc Nephrol 31 1479-1495 (2020)
PMID: 32540856

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129066 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-actinin-4 Chain: A
Molecule details ›
Chain: A
Length: 234 amino acids
Theoretical weight: 26.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O43707 (Residues: 47-271; Coverage: 25%)
Gene name: ACTN4
Sequence domains: Calponin homology (CH) domain

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 45.589Å b: 61.732Å c: 89.534Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.136 0.134 0.172
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Abnormal Crosstalk between Endothelial Cells and Podocytes Mediates Tyrosine Kinase Inhibitor (TKI)-Induced Nephrotoxicity.
Gu et al. (2021)
4 more

3 mentions without citation

Pathogenic SPTBN1 variants cause an autosomal dominant neurodevelopmental syndrome.
Cousin et al. (2021)
2 more