› 6obd

X-ray diffraction
2.2Å resolution

Crystal structure of anti-GLD52 Fab complex with human GLD52 peptide mimetic

Released:
DOI: 10.2210/pdb6obd/pdb
PDB entry 6obd coloured by chain, front view.
PDB entry 6obd coloured by chain, side view.
PDB entry 6obd coloured by chain, top view.
Source organisms:
Primary publication:
Engineering an anti-CD52 antibody for enhanced deamidation stability.
Qiu H, Wei R, Jaworski J, Boudanova E, Hughes H, VanPatten S, Lund A, Day J, Zhou Y, McSherry T, Pan CQ, Sendak R
OpenAccess logo MAbs 11 1266-1275 (2019)
PMID: 31199181

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211029 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
anti-GLD52 Fab light chain Chains: A, L
Molecule details ›
Chains: A, L
Length: 216 amino acids
Theoretical weight: 23.6 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
anti-GLD52 Fab heavy chain Chains: B, H
Molecule details ›
Chains: B, H
Length: 216 amino acids
Theoretical weight: 23.1 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
CAMPATH-1 antigen Chains: E, F
Molecule details ›
Chains: E, F
Length: 10 amino acids
Theoretical weight: 1.02 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P31358 (Residues: 27-36; Coverage: 27%)
Gene names: CD52, CDW52, HE5

Ligands and Environments

2 bound ligands:
Ligand ZN 5 x ZN
Ligand OPE 2 x OPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: Cu FINE FOCUS
Spacegroup: P212121
Unit cell:
a: 54.4Å b: 131.2Å c: 133.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.234 0.307
Expression systems:
  • Cricetulus griseus
  • Not provided

Quick links

Citations

3 review citations

Antibody Structure and Function: The Basis for Engineering Therapeutics.
Chiu et al. (2019)
2 more

2 mentions without citation

Construction of a solid Cox model for AML patients based on multiomics bioinformatic analysis.
Li et al. (2022)
1 more