6obj

Electron Microscopy
3.5Å resolution

Structure of a DNA-bound dimer extracted from filamentous SgrAI endonuclease in its activated form

Released:
Source organism: Streptomyces griseus
Primary publication:
Mechanism of Filamentation-Induced Allosteric Activation of the SgrAI Endonuclease.
Structure 27 1497-1507.e3 (2019)
PMID: 31447289
Related structures: EMD-20015

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 40-mer (preferred)
PDBe Complex ID:
PDB-CPX-120377 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
SgraIR restriction enzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 339 amino acids
Theoretical weight: 38.07 KDa
Source organism: Streptomyces griseus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9F6L0 (Residues: 1-339; Coverage: 100%)
Gene name: sgraIR
Sequence domains: Cfr10I/Bse634I restriction endonuclease
DNA (26-MER) Chains: C, D
Molecule details ›
Chains: C, D
Length: 40 nucleotides
Theoretical weight: 12.31 KDa
Source organism: Streptomyces griseus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-20015
Expression systems:
  • Escherichia coli
  • Not provided