› 6oel

X-ray diffraction
3.1Å resolution

Engineered Fab bound to IL-4 receptor

Released:
DOI: 10.2210/pdb6oel/pdb
PDB entry 6oel coloured by chain, front view.
PDB entry 6oel coloured by chain, side view.
PDB entry 6oel coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A strategy for the selection of monovalent antibodies that span protein dimer interfaces.
Spangler JB, Moraga I, Jude KM, Savvides CS, Garcia KC
OpenAccess logo J Biol Chem 294 13876-13886 (2019)
PMID: 31387945

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-231713 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (6 distinct):
Interleukin-4 Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.98 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P05112 (Residues: 25-153; Coverage: 100%)
Gene name: IL4
Sequence domains: Interleukin 4
Soluble interleukin-4 receptor subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 202 amino acids
Theoretical weight: 22.96 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P24394 (Residues: 27-224; Coverage: 25%)
Gene names: 582J2.1, IL4R, IL4RA
Sequence domains: Interleukin-4 receptor alpha chain, N-terminal
Cytokine receptor common subunit gamma Chain: C
Molecule details ›
Chain: C
Length: 189 amino acids
Theoretical weight: 22.82 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P31785 (Residues: 61-249; Coverage: 55%)
  • Best match: P31785-2 (Residues: 9-59)
Gene name: IL2RG
Sequence domains:
engineered Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 229 amino acids
Theoretical weight: 24.63 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
engineered Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 224 amino acids
Theoretical weight: 24.39 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
2 bound ligands:
Ligand NAG 5 x NAG
Ligand NO3 1 x NO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: F4132
Unit cell:
a: 328.1Å b: 328.1Å c: 328.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.199 0.256
Expression system: Trichoplusia ni

Quick links

Citations

3 review citations

A comprehensive comparison between camelid nanobodies and single chain variable fragments.
Asaadi et al. (2021)
2 more

1 mention without citation

A strategy for the selection of monovalent antibodies that span protein dimer interfaces.
Spangler et al. (2019)