› 6ofe

Electron Microscopy
3.61Å resolution

Helical reconstruction of Type III Secretion System Needle filament mutant-PrgI S49A

Released:
DOI: 10.2210/pdb6ofe/pdb
PDB entry 6ofe coloured by chain, front view.
PDB entry 6ofe coloured by chain, side view.
PDB entry 6ofe coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344
Primary publication:
A polymorphic helix of a Salmonella needle protein relays signals defining distinct steps in type III secretion.
Guo EZ, Desrosiers DC, Zalesak J, Tolchard J, Berbon M, Habenstein B, Marlovits T, Loquet A, Galán JE
OpenAccess logo PLoS Biol 17 e3000351 (2019)
PMID: 31260457
Related structures: EMD-20043

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo icosamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154441 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SPI-1 type 3 secretion system needle filament protein Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T
Length: 83 amino acids
Theoretical weight: 9.13 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P41784 (Residues: 1-80; Coverage: 100%)
Gene names: STM2873, prgI, sctF1
Sequence domains: Type III secretion needle MxiH, YscF, SsaG, EprI, PscF, EscF

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.61Å
Relevant EMDB volumes: EMD-20043
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Solid-State NMR Investigations of Extracellular Matrixes and Cell Walls of Algae, Bacteria, Fungi, and Plants.
Ghassemi et al. (2022)
3 more