› 6ogc

X-ray diffraction
1.18Å resolution

Crystal structure of Green Fluorescent Protein (GFP); S65T, Y66(2,3-F2Y), H148D; circular permutant (50-51)

Released:
DOI: 10.2210/pdb6ogc/pdb
PDB entry 6ogc coloured by chain, front view.
PDB entry 6ogc coloured by chain, side view.
PDB entry 6ogc coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Unusual Spectroscopic and Electric Field Sensitivity of Chromophores with Short Hydrogen Bonds: GFP and PYP as Model Systems.
Lin CY, Boxer SG
J Phys Chem B 124 9513-9525 (2020)
PMID: 33073990

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 251 amino acids
Theoretical weight: 28.1 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 49-237; Coverage: 79%)
Gene name: GFP
Sequence domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MJ1 2 x MJ1

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 52.275Å b: 68.866Å c: 60.968Å
α: 90° β: 100.34° γ: 90°
R-values:
R R work R free
0.144 0.143 0.161
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

Energetic Basis and Design of Enzyme Function Demonstrated Using GFP, an Excited-State Enzyme.
Lin et al. (2022)
3 more

1 mention without citation

Unusual Spectroscopic and Electric Field Sensitivity of Chromophores with Short Hydrogen Bonds: GFP and PYP as Model Systems.
Lin et al. (2020)