› 6ol7

X-ray diffraction
2.42Å resolution

Crystal structure of glVRC01 scFv in complex with anti-idiotype iv8 scFv

Released:
DOI: 10.2210/pdb6ol7/pdb
PDB entry 6ol7 coloured by chain, front view.
PDB entry 6ol7 coloured by chain, side view.
PDB entry 6ol7 coloured by chain, top view.
Source organisms:
Primary publication:
Anti-idiotypic antibodies elicit anti-HIV-1-specific B cell responses.
Dosenovic P, Pettersson AK, Wall A, Thientosapol ES, Feng J, Weidle C, Bhullar K, Kara EE, Hartweger H, Pai JA, Gray MD, Parks KR, Taylor JJ, Pancera M, Stamatatos L, Nussenzweig MC, McGuire AT
OpenAccess logo J Exp Med 216 2316-2330 (2019)
PMID: 31345931

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-232236 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
iv8 Heavy Chain Chains: A, E, K, P
Molecule details ›
Chains: A, E, K, P
Length: 131 amino acids
Theoretical weight: 14.53 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
glVRC01 Light Chain Chains: B, F, L, N
Molecule details ›
Chains: B, F, L, N
Length: 111 amino acids
Theoretical weight: 11.67 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
glVRC01 Heavy Chain Chains: C, H, I, M
Molecule details ›
Chains: C, H, I, M
Length: 141 amino acids
Theoretical weight: 15.61 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
iv8 Light Chain Chains: D, J, O, Q
Molecule details ›
Chains: D, J, O, Q
Length: 125 amino acids
Theoretical weight: 13.33 KDa
Source organism: Mus musculus
Expression system: Homo sapiens

Ligands and Environments

3 bound ligands:
Ligand EDO 28 x EDO
Ligand PEG 10 x PEG
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P1
Unit cell:
a: 51.583Å b: 107.937Å c: 116.961Å
α: 85.49° β: 80.28° γ: 84.17°
R-values:
R R work R free
0.177 0.174 0.224
Expression system: Homo sapiens

Quick links

Citations

3 review citations

Public Immunity: Evolutionary Spandrels for Pathway-Amplifying Protective Antibodies.
Sangesland et al. (2021)
2 more

1 mention without citation

Development of a VRC01-class germline targeting immunogen derived from anti-idiotypic antibodies.
Seydoux et al. (2021)