› 6olk

Electron Microscopy
4Å resolution

CryoEM structure of PilT4 from Geobacter metallireducens without adding nucleotide: C3ocococ conformation

Released:
DOI: 10.2210/pdb6olk/pdb
PDB entry 6olk coloured by chain, front view.
PDB entry 6olk coloured by chain, side view.
PDB entry 6olk coloured by chain, top view.
Source organism: Geobacter metallireducens GS-15
Primary publication:
Multiple conformations facilitate PilT function in the type IV pilus.
McCallum M, Benlekbir S, Nguyen S, Tammam S, Rubinstein JL, Burrows LL, Howell PL
OpenAccess logo Nat Commun 10 5198 (2019)
PMID: 31729381
Related structures: EMD-20115

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174486 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacterial type II secretion system protein E domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 385 amino acids
Theoretical weight: 42.88 KDa
Source organism: Geobacter metallireducens GS-15
Expression system: Escherichia coli
UniProt:
  • Canonical: Q39VU6 (Residues: 1-365; Coverage: 100%)
Gene names: Gmet_1394, pilT-4
Sequence domains: Type II/IV secretion system protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4Å
Relevant EMDB volumes: EMD-20115
Expression system: Escherichia coli

Quick links

Citations

8 citation in other articles

PilT and PilU are homohexameric ATPases that coordinate to retract type IVa pili.
Chlebek et al. (2019)
7 more