6orl

Electron Microscopy
3.5Å resolution

RF1 pre-accommodated 70S complex at 24 ms

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 55-mer (preferred)
PDBe Complex ID:
PDB-CPX-101190 (preferred)
Entry contents:
50 distinct polypeptide molecules
5 distinct RNA molecules
Macromolecules (55 distinct):
Large ribosomal subunit protein uL2 Chain: B
Molecule details ›
Chain: B
Length: 271 amino acids
Theoretical weight: 29.66 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein uL3 Chain: C
Molecule details ›
Chain: C
Length: 209 amino acids
Theoretical weight: 22.28 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein uL4 Chain: D
Molecule details ›
Chain: D
Length: 201 amino acids
Theoretical weight: 22.12 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein uL5 Chain: E
Molecule details ›
Chain: E
Length: 177 amino acids
Theoretical weight: 20.07 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein uL6 Chain: F
Molecule details ›
Chain: F
Length: 175 amino acids
Theoretical weight: 18.67 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein bL9 Chain: G
Molecule details ›
Chain: G
Length: 149 amino acids
Theoretical weight: 15.79 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein uL13 Chain: J
Molecule details ›
Chain: J
Length: 142 amino acids
Theoretical weight: 16.05 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein uL14 Chain: K
Molecule details ›
Chain: K
Length: 123 amino acids
Theoretical weight: 13.57 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein uL15 Chain: L
Molecule details ›
Chain: L
Length: 144 amino acids
Theoretical weight: 15.01 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein uL16 Chain: M
Molecule details ›
Chain: M
Length: 136 amino acids
Theoretical weight: 15.31 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein bL17 Chain: N
Molecule details ›
Chain: N
Length: 119 amino acids
Theoretical weight: 13.59 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein uL18 Chain: O
Molecule details ›
Chain: O
Length: 116 amino acids
Theoretical weight: 12.66 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein bL19 Chain: P
Molecule details ›
Chain: P
Length: 114 amino acids
Theoretical weight: 13.03 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein bL20 Chain: Q
Molecule details ›
Chain: Q
Length: 117 amino acids
Theoretical weight: 13.4 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein bL21 Chain: R
Molecule details ›
Chain: R
Length: 103 amino acids
Theoretical weight: 11.59 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein uL22 Chain: S
Molecule details ›
Chain: S
Length: 110 amino acids
Theoretical weight: 12.25 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein uL23 Chain: T
Molecule details ›
Chain: T
Length: 94 amino acids
Theoretical weight: 10.66 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein uL24 Chain: U
Molecule details ›
Chain: U
Length: 103 amino acids
Theoretical weight: 11.21 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein bL25 Chain: V
Molecule details ›
Chain: V
Length: 94 amino acids
Theoretical weight: 10.71 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein bL27 Chain: W
Molecule details ›
Chain: W
Length: 76 amino acids
Theoretical weight: 8.28 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein bL28 Chain: X
Molecule details ›
Chain: X
Length: 77 amino acids
Theoretical weight: 8.9 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein uL29 Chain: Y
Molecule details ›
Chain: Y
Length: 62 amino acids
Theoretical weight: 7.16 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein uL30 Chain: Z
Molecule details ›
Chain: Z
Length: 58 amino acids
Theoretical weight: 6.42 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein bL32 Chain: b
Molecule details ›
Chain: b
Length: 56 amino acids
Theoretical weight: 6.33 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein bL33 Chain: c
Molecule details ›
Chain: c
Length: 52 amino acids
Theoretical weight: 6.06 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein bL34 Chain: d
Molecule details ›
Chain: d
Length: 46 amino acids
Theoretical weight: 5.4 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein bL35 Chain: e
Molecule details ›
Chain: e
Length: 64 amino acids
Theoretical weight: 7.18 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein bL36 Chain: f
Molecule details ›
Chain: f
Length: 38 amino acids
Theoretical weight: 4.38 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein uS2 Chain: g
Molecule details ›
Chain: g
Length: 225 amino acids
Theoretical weight: 25.07 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein uS3 Chain: h
Molecule details ›
Chain: h
Length: 208 amino acids
Theoretical weight: 23.25 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein uS4 Chain: i
Molecule details ›
Chain: i
Length: 205 amino acids
Theoretical weight: 23.38 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein uS5 Chain: j
Molecule details ›
Chain: j
Length: 156 amino acids
Theoretical weight: 16.48 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein bS6, non-modified isoform Chain: k
Molecule details ›
Chain: k
Length: 104 amino acids
Theoretical weight: 12.13 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein uS7 Chain: l
Molecule details ›
Chain: l
Length: 151 amino acids
Theoretical weight: 16.86 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein uS8 Chain: m
Molecule details ›
Chain: m
Length: 129 amino acids
Theoretical weight: 14.02 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein uS9 Chain: n
Molecule details ›
Chain: n
Length: 127 amino acids
Theoretical weight: 14.55 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein uS10 Chain: o
Molecule details ›
Chain: o
Length: 99 amino acids
Theoretical weight: 11.25 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein uS11 Chain: p
Molecule details ›
Chain: p
Length: 117 amino acids
Theoretical weight: 12.49 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein uS12 Chain: q
Molecule details ›
Chain: q
Length: 123 amino acids
Theoretical weight: 13.68 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein uS13 Chain: r
Molecule details ›
Chain: r
Length: 116 amino acids
Theoretical weight: 12.87 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein uS14 Chain: s
Molecule details ›
Chain: s
Length: 100 amino acids
Theoretical weight: 11.48 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein uS15 Chain: t
Molecule details ›
Chain: t
Length: 88 amino acids
Theoretical weight: 10.16 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein bS16 Chain: u
Molecule details ›
Chain: u
Length: 82 amino acids
Theoretical weight: 9.21 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein uS17 Chain: v
Molecule details ›
Chain: v
Length: 80 amino acids
Theoretical weight: 9.26 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein bS18 Chain: w
Molecule details ›
Chain: w
Length: 66 amino acids
Theoretical weight: 7.73 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein uS19 Chain: x
Molecule details ›
Chain: x
Length: 83 amino acids
Theoretical weight: 9.42 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein bS20 Chain: y
Molecule details ›
Chain: y
Length: 86 amino acids
Theoretical weight: 9.58 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein bS21 Chain: z
Molecule details ›
Chain: z
Length: 70 amino acids
Theoretical weight: 8.39 KDa
Source organism: Escherichia coli
FME-PHE-PHE Chain: H
Molecule details ›
Chain: H
Length: 3 amino acids
Theoretical weight: 472 Da
Source organism: Escherichia coli
Peptide chain release factor RF1 Chain: A
Molecule details ›
Chain: A
Length: 257 amino acids
Theoretical weight: 28.5 KDa
Source organism: Escherichia coli
23S ribosomal RNA Chain: 1
Molecule details ›
Chain: 1
Length: 2903 nucleotides
Theoretical weight: 941.53 KDa
Source organism: Escherichia coli
Sequence domains: Bacterial large subunit ribosomal RNA
16S ribosomal RNA Chain: 2
Molecule details ›
Chain: 2
Length: 1534 nucleotides
Theoretical weight: 497.41 KDa
Source organism: Escherichia coli
Sequence domains: Bacterial small subunit ribosomal RNA
5S ribosomal RNA Chain: 3
Molecule details ›
Chain: 3
Length: 120 nucleotides
Theoretical weight: 38.79 KDa
Source organism: Escherichia coli
Sequence domains: 5S ribosomal RNA
P-tRNA Chain: 5
Molecule details ›
Chain: 5
Length: 76 nucleotides
Theoretical weight: 24.57 KDa
Source organism: Escherichia coli
Sequence domains: tRNA
Messenger RNA Chain: 4
Molecule details ›
Chain: 4
Length: 9 nucleotides
Theoretical weight: 2.75 KDa
Source organism: Escherichia coli

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-20174