› 6os4

X-ray diffraction
2.05Å resolution

Calmodulin in complex with farnesyl cysteine methyl ester

Released:
DOI: 10.2210/pdb6os4/pdb
PDB entry 6os4 coloured by chain, front view.
PDB entry 6os4 coloured by chain, side view.
PDB entry 6os4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Calmodulin disrupts plasma membrane localization of farnesylated KRAS4b by sequestering its lipid moiety.
Grant BMM, Enomoto M, Back SI, Lee KY, Gebregiworgis T, Ishiyama N, Ikura M, Marshall CB
Sci Signal 13 (2020)
PMID: 32234958

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-143908 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calmodulin-1 Chain: A
Molecule details ›
Chain: A
Length: 148 amino acids
Theoretical weight: 16.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DP23 (Residues: 2-149; Coverage: 99%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair

Ligands and Environments

2 bound ligands:
Ligand CA 4 x CA
Ligand 5U0 1 x 5U0
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P6122
Unit cell:
a: 40.379Å b: 40.379Å c: 338.137Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.191 0.225
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Mechanisms of Ras Membrane Organization and Signaling: Ras Rocks Again.
Abankwa et al. (2020)
3 more

3 mentions without citation

A Non-Canonical Calmodulin Target Motif Comprising a Polybasic Region and Lipidated Terminal Residue Regulates Localization.
Grant et al. (2020)
2 more