› 6ot0

Electron Microscopy
3.84Å resolution

Structure of human Smoothened-Gi complex

Released:
DOI: 10.2210/pdb6ot0/pdb
PDB entry 6ot0 coloured by chain, front view.
PDB entry 6ot0 coloured by chain, side view.
PDB entry 6ot0 coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structure of oxysterol-bound human Smoothened coupled to a heterotrimeric Gi.
Qi X, Liu H, Thompson B, McDonald J, Zhang C, Li X
Nature 571 279-283 (2019)
PMID: 31168089
Related structures: EMD-20190

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-211382 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Protein smoothened Chain: R
Molecule details ›
Chain: R
Length: 563 amino acids
Theoretical weight: 62.11 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q99835 (Residues: 1-555; Coverage: 70%)
Gene names: SMO, SMOH
Sequence domains:
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.41 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 351 amino acids
Theoretical weight: 38.74 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.26 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 239 amino acids
Theoretical weight: 25.79 KDa
Source organism: Mus musculus
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand CO1 1 x CO1
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.84Å
Relevant EMDB volumes: EMD-20190
Expression systems:
  • Homo sapiens
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

19 review citations

Hedgehog signaling mechanism and role in cancer.
Jiang J. (2022)
18 more

25 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
24 more