› 6ot8

X-ray diffraction
1.5Å resolution

Bimetallic hexameric cage design 4 (BMC4) from cytochrome cb562

Released:
DOI: 10.2210/pdb6ot8/pdb
PDB entry 6ot8 coloured by chain, front view.
PDB entry 6ot8 coloured by chain, side view.
PDB entry 6ot8 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Constructing protein polyhedra via orthogonal chemical interactions.
Golub E, Subramanian RH, Esselborn J, Alberstein RG, Bailey JB, Chiong JA, Yan X, Booth T, Baker TS, Tezcan FA
OpenAccess logo Nature 578 172-176 (2020)
PMID: 31969701

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142159 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble cytochrome b562 Chain: A
Molecule details ›
Chain: A
Length: 106 amino acids
Theoretical weight: 11.8 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene name: cybC
Sequence domains: Cytochrome b562

Ligands and Environments

4 bound ligands:
Ligand HEC 1 x HEC
Ligand ZN 3 x ZN
Ligand FE 1 x FE
Ligand HAE 1 x HAE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P6322
Unit cell:
a: 87Å b: 87Å c: 63.3Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.19 0.218
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Enzymatic Noncovalent Synthesis.
He et al. (2020)
5 more

1 mention without citation

Constructing protein polyhedra via orthogonal chemical interactions.
Golub et al. (2020)