› 6ovl

X-ray diffraction
2.1Å resolution

2.1 Angstrom structure of wild type Glyoxylate/Hydroxypyruvate reductase A from Escherichia Coli in complex with glyoxylate and NADP

Released:
DOI: 10.2210/pdb6ovl/pdb
PDB entry 6ovl coloured by chain, front view.
PDB entry 6ovl coloured by chain, side view.
PDB entry 6ovl coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Vuksanovic N, Silvaggi NR

Function and Biology Details

Reactions catalysed: 
Glycolate + NADP(+) = glyoxylate + NADPH
D-glycerate + NAD(P)(+) = hydroxypyruvate + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160021 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glyoxylate/hydroxypyruvate reductase A Chain: A
Molecule details ›
Chain: A
Length: 318 amino acids
Theoretical weight: 36.19 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P75913 (Residues: 1-312; Coverage: 100%)
Gene names: JW5146, b1033, ghrA, ycdW
Sequence domains: D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP
5 bound ligands:
Ligand GOL 1 x GOL
Ligand PO4 2 x PO4
Ligand GLV 1 x GLV
Ligand K 1 x K
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P6522
Unit cell:
a: 158.82Å b: 158.82Å c: 96.71Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.165 0.164 0.184

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