› 6ox1

X-ray diffraction
1.95Å resolution

SETD3 in Complex with an Actin Peptide with Target Histidine Partially Methylated

Released:
DOI: 10.2210/pdb6ox1/pdb
Model geometry
Fit model/data
PDB entry 6ox1 coloured by chain, front view.
PDB entry 6ox1 coloured by chain, side view.
PDB entry 6ox1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the target specificity of actin histidine methyltransferase SETD3.
Dai S, Horton JR, Woodcock CB, Wilkinson AW, Zhang X, Gozani O, Cheng X
OpenAccess logo Nat Commun 10 3541 (2019)
PMID: 31388018

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + protein L-histidine = S-adenosyl-L-homocysteine + protein N(tau)-methyl-L-histidine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158074 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, cytoplasmic 1, N-terminally processed Chains: Y, Z
Molecule details ›
Chains: Y, Z
Length: 15 amino acids
Theoretical weight: 1.8 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P60709 (Residues: 66-80; Coverage: 4%)
Gene name: ACTB
Actin-histidine N-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 599 amino acids
Theoretical weight: 67.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q86TU7 (Residues: 1-594; Coverage: 100%)
Gene names: C14orf154, SETD3
Sequence domains:

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH
2 bound ligands:
Ligand GOL 1 x GOL
Ligand EDO 22 x EDO
1 modified residue:
Ligand HIC 2 x HIC

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 60.308Å b: 175.865Å c: 66.967Å
α: 90° β: 92.38° γ: 90°
R-values:
R R work R free
0.171 0.17 0.201
Expression systems:
  • Not provided
  • Escherichia coli

Quick links

Citations

4 review citations

Regulation of actin isoforms in cellular and developmental processes.
Kashina AS. (2020)
3 more

1 mention without citation

Structural basis for the target specificity of actin histidine methyltransferase SETD3.
Dai et al. (2019)

PDB-REDO

The sliders below show the change in model quality between original PDB entry and the PDB-REDO entry

Model Geometry
 
 
Data not available
DNA/RNA Geometry
 
 
Data not available
Fit model/data
 
 
Data not available
PDB-REDO