› 6ozb

X-ray diffraction
1.8Å resolution

Crystal structure of the phycoerythrobilin-bound GAF domain from a cyanobacterial phytochrome

Released:
DOI: 10.2210/pdb6ozb/pdb
PDB entry 6ozb coloured by chain, front view.
PDB entry 6ozb coloured by chain, side view.
PDB entry 6ozb coloured by chain, top view.
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Primary publication:
The interplay between chromophore and protein determines the extended excited state dynamics in a single-domain phytochrome.
Slavov C, Fischer T, Barnoy A, Shin H, Rao AG, Wiebeler C, Zeng X, Sun Y, Xu Q, Gutt A, Zhao KH, Gärtner W, Yang X, Schapiro I, Wachtveitl J
Proc Natl Acad Sci U S A 117 16356-16362 (2020)
PMID: 32591422

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187117 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 207 amino acids
Theoretical weight: 23 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8YTL8 (Residues: 1-200; Coverage: 22%)
Gene name: all2699
Sequence domains: GAF domain

Ligands and Environments

1 bound ligand:
Ligand PEB 3 x PEB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P3121
Unit cell:
a: 78.967Å b: 78.967Å c: 209.299Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.153 0.152 0.194
Expression system: Escherichia coli

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Citations

14 citation in other articles

All-Red-Light Photoswitching of Indirubin Controlled by Supramolecular Interactions.
Thumser et al. (2021)
13 more

3 mentions without citation

Computational Methods in Immunology and Vaccinology: Design and Development of Antibodies and Immunogens.
Guarra et al. (2023)
2 more