› 6p1g

X-ray diffraction
2.05Å resolution

Copper-bound PCuAC domain from PmoF2

Released:
DOI: 10.2210/pdb6p1g/pdb
PDB entry 6p1g coloured by chain, front view.
PDB entry 6p1g coloured by chain, side view.
PDB entry 6p1g coloured by chain, top view.
Source organism: Methylosinus trichosporium OB3b
Primary publication:
PCuAC domains from methane-oxidizing bacteria use a histidine brace to bind copper.
Fisher OS, Sendzik MR, Ross MO, Lawton TJ, Hoffman BM, Rosenzweig AC
J Biol Chem 294 16351-16363 (2019)
PMID: 31527086

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104196 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Copper chaperone PCu(A)C Chains: A, B
Molecule details ›
Chains: A, B
Length: 133 amino acids
Theoretical weight: 14.63 KDa
Source organism: Methylosinus trichosporium OB3b
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A2D2CWX5 (Residues: 29-159; Coverage: 68%)
Gene name: CQW49_04640
Sequence domains: Copper chaperone PCu(A)C

Ligands and Environments

2 bound ligands:
Ligand CU 2 x CU
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P3221
Unit cell:
a: 89.35Å b: 89.35Å c: 60.07Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.186 0.229
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Orchestrating copper binding: structure and variations on the cupredoxin fold.
Guo et al. (2022)
2 other articles

1 mention without citation

PCuAC domains from methane-oxidizing bacteria use a histidine brace to bind copper.
Fisher et al. (2019)