› 6p29

X-ray diffraction
1.5Å resolution

N-demethylindolmycin synthase (PluN2) in complex with N-demethylindolmycin

Released:
DOI: 10.2210/pdb6p29/pdb
PDB entry 6p29 coloured by chain, front view.
PDB entry 6p29 coloured by chain, side view.
PDB entry 6p29 coloured by chain, top view.
Source organism: Pseudoalteromonas luteoviolacea
Primary publication:
Convergent biosynthetic transformations to a bacterial specialized metabolite.
Du YL, Higgins MA, Zhao G, Ryan KS
Nat Chem Biol 15 1043-1048 (2019)
PMID: 31406372

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103090 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
VOC domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 140 amino acids
Theoretical weight: 16.38 KDa
Source organism: Pseudoalteromonas luteoviolacea
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A167HII1 (Residues: 1-132; Coverage: 100%)
Gene name: N473_05385
Sequence domains: Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily

Ligands and Environments

2 bound ligands:
Ligand NQ7 2 x NQ7
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P41212
Unit cell:
a: 54.07Å b: 54.07Å c: 176.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.168 0.196
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

A shared mechanistic pathway for pyridoxal phosphate-dependent arginine oxidases.
Hoffarth et al. (2021)
1 more