› 6p58

X-ray diffraction
1.5Å resolution

Dark and Steady State-Illuminated Crystal Structure of Cyanobacteriochrome Receptor PixJ at 150K

Released:
DOI: 10.2210/pdb6p58/pdb
PDB entry 6p58 coloured by chain, front view.
PDB entry 6p58 coloured by chain, side view.
PDB entry 6p58 coloured by chain, top view.
Source organism: Thermosynechococcus vestitus BP-1
Primary publication:
Photoreversible interconversion of a phytochrome photosensory module in the crystalline state.
Burgie ES, Clinger JA, Miller MD, Brewster AS, Aller P, Butryn A, Fuller FD, Gul S, Young ID, Pham CC, Kim IS, Bhowmick A, O'Riordan LJ, Sutherlin KD, Heinemann JV, Batyuk A, Alonso-Mori R, Hunter MS, Koglin JE, Yano J, Yachandra VK, Sauter NK, Cohen AE, Kern J, Orville AM, Phillips GN, Vierstra RD
OpenAccess logo Proc Natl Acad Sci U S A 117 300-307 (2020)
PMID: 31852825

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-568173 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyl-accepting chemotaxis protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 150 amino acids
Theoretical weight: 17.15 KDa
Source organism: Thermosynechococcus vestitus BP-1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8DLC7 (Residues: 435-584; Coverage: 16%)
Gene name: tll0569
Sequence domains: GAF domain

Ligands and Environments

3 bound ligands:
Ligand VRB 2 x VRB
Ligand MG 4 x MG
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 41.618Å b: 61.052Å c: 116.148Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.159 0.196
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The Red Edge: Bilin-Binding Photoreceptors as Optogenetic Tools and Fluorescence Reporters.
Tang et al. (2021)
2 more

1 mention without citation

Photoreversible interconversion of a phytochrome photosensory module in the crystalline state.
Burgie et al. (2020)